7ZG6
TacA1 antitoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | H 3 |
| Unit cell lengths | 68.990, 68.990, 69.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.490 - 1.950 |
| R-factor | 0.1999 |
| Rwork | 0.197 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ak7 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.049 | 1.076 |
| Rmeas | 0.055 | |
| Rpim | 0.024 | 0.520 |
| Number of reflections | 9080 | 475 |
| <I/σ(I)> | 14.3 | 1.4 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 5 | 5.2 |
| CC(1/2) | 0.999 | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1% Tryptone 1mM Sodium azide 50mM HEPES pH 7.0 12% PEG3350 |
