7ZET
Crystal structure of human Clusterin, crystal form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.77490 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.742, 43.808, 102.800 |
Unit cell angles | 90.00, 107.29, 90.00 |
Refinement procedure
Resolution | 27.610 - 2.800 |
R-factor | 0.2346 |
Rwork | 0.232 |
R-free | 0.27420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlphaFold AF-P10909-F1-model_v1.pdb |
Data reduction software | XDS (VERSION Feb 5, 2021) |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP (11.4.06) |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.080 | 49.080 | 2.950 |
High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
Rmerge | 0.146 | 0.041 | 1.656 |
Rmeas | 0.172 | 0.049 | 1.942 |
Rpim | 0.088 | 0.026 | 1.000 |
Total number of observations | 50668 | 1471 | 7108 |
Number of reflections | 13861 | 463 | 1966 |
<I/σ(I)> | 5.9 | 19.4 | 0.7 |
Completeness [%] | 98.2 | 95.2 | 96.1 |
Redundancy | 3.7 | 3.2 | 3.6 |
CC(1/2) | 0.995 | 0.997 | 0.417 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 30% PEG-4000, 0.2 M NH4-acetate, 0.1 M Na-citrate pH 5.6 |