7ZCB

Human Pikachurin/EGFLAM N-terminal Fibronectin-III (1-2) domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.873
Spacegroup name	C 1 2 1
Unit cell lengths	70.902, 62.077, 106.863
Unit cell angles	90.00, 104.28, 90.00

Refinement procedure

Resolution	46.060 - 2.500
R-factor	0.2454
Rwork	0.244
R-free	0.27710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fm4
RMSD bond length	0.005
RMSD bond angle	0.845
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.060	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.130	1.594
Rpim	0.118	1.423
Number of reflections	15494	1726
<I/σ(I)>	6.1	0.8
Completeness [%]	98.8	98.9
Redundancy	3.7	4
CC(1/2)	0.994	0.519

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	18% PEG 3350, 180 mM Sodium Bromide