7ZC9

Human Pikachurin/EGFLAM C-terminal Laminin-G domain (LG3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	C 2 2 21
Unit cell lengths	73.805, 103.818, 133.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.380 - 2.100
R-factor	0.2085
Rwork	0.207
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sh4
RMSD bond length	0.003
RMSD bond angle	0.644
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.380	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.098	1.276
Rpim	0.058	0.742
Number of reflections	30331	2451
<I/σ(I)>	11.9	1.7
Completeness [%]	100.0	100
Redundancy	7.3	7.6
CC(1/2)	0.999	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	1.5-2.0 M ammonium sulfate 100 mM sodium acetate pH 4.6