7ZC8
Crystal structure of the C-terminal domain of FusB, a TonB homologue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 |
| Unit cell lengths | 94.661, 94.661, 58.246 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.530 - 2.080 |
| R-factor | 0.2131 |
| Rwork | 0.212 |
| R-free | 0.23958 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gsk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.244 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 2.140 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 18033 | 1406 |
| <I/σ(I)> | 36.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.2 | |
| CC(1/2) | 0.999 | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | Tris pH 8.5, 8% PEG8000 |
