7ZBR

Crystal Structure of full-length Bartonella Effector protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	70.492, 70.492, 213.961
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.380 - 3.000
R-factor	0.2734
Rwork	0.270
R-free	0.30300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nps 4yk3
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.380	3.060
High resolution limit [Å]	3.000	3.000
Number of reflections	13003	1271
<I/σ(I)>	8.1
Completeness [%]	99.6
Redundancy	12.8
CC(1/2)	0.993	0.335

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293.15	100 mM Hepes pH 7.8, 0.175 mM LiCl and 20% v/v PEG 8000