7ZBO
Amine Dehydrogenase MATOUAmDH2 in complex with NADP+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2020-02-20 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.098, 112.195, 153.146 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.210 - 2.320 |
R-factor | 0.2399 |
Rwork | 0.237 |
R-free | 0.28780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iau |
RMSD bond length | 0.008 |
RMSD bond angle | 1.633 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.210 | 2.370 |
High resolution limit [Å] | 2.320 | 2.320 |
Rmerge | 0.260 | 1.370 |
Rpim | 0.140 | 0.740 |
Number of reflections | 68673 | 4580 |
<I/σ(I)> | 5.8 | 1.6 |
Completeness [%] | 100.0 | |
Redundancy | 8.4 | |
CC(1/2) | 0.990 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M ammonium sulfate; 0.1 M bis-tris propane; 25% (w/v) PEG3350; 10% (v/v) methylpentanediol |