7ZBC
Dark state crystal structure of bovine rhodopsin in Lipidic Cubic Phase (SACLA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2018-11-01 |
| Detector | MPCCD |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 61.290, 90.810, 150.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.470 - 1.800 |
| R-factor | 0.1983 |
| Rwork | 0.198 |
| R-free | 0.22320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u19 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.958 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.470 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 78209 | 7715 |
| <I/σ(I)> | 8.87 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1342.8 | 963.9 |
| CC(1/2) | 0.998 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 9 | 294 | 36 % PEG 600, 100 mM Bicine pH 9.0 |
