7ZAW

GPC3-Unc5D octamer structure and role in cell migration

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-08
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.92010
Spacegroup name	P 31 2 1
Unit cell lengths	100.190, 100.190, 90.186
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.090 - 2.580
R-factor	0.2329
Rwork	0.230
R-free	0.28490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ywt
RMSD bond length	0.004
RMSD bond angle	0.679
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.860	2.650
High resolution limit [Å]	2.580	2.580
Rmerge	0.075
Rmeas	0.079
Rpim	0.020	1.390
Number of reflections	16895	1239
<I/σ(I)>	16	0.9
Completeness [%]	100.0	100
Redundancy	15.5	6.9
CC(1/2)	0.999	0.324

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	20% ethylene glycol, 10% w/v PEG 8000, 0.1 M Tris/BICINE (pH 8.5) and 0.02 M of amino acids (L-Na-glutamate, alanine, glycine, lysine-HCl, and serine)