7ZAV

GPC3-Unc5D octamer structure and role in cell migration

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96859
Spacegroup name	P 31 2 1
Unit cell lengths	100.488, 100.488, 90.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.940 - 2.900
R-factor	0.2203
Rwork	0.218
R-free	0.26150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ywt
RMSD bond length	0.004
RMSD bond angle	0.662
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.760	2.980
High resolution limit [Å]	2.900	2.900
Rmerge	0.130
Rmeas	0.143
Rpim	0.065	0.999
Number of reflections	12106	875
<I/σ(I)>	9.2	1.1
Completeness [%]	100.0	100
Redundancy	9.5	9.6
CC(1/2)	0.997	0.493

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M ammonium nitrate and 20% w/v PEG3350