7ZA5
GSTF sh101 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.450, 46.382, 110.822 |
| Unit cell angles | 90.00, 112.04, 90.00 |
Refinement procedure
| Resolution | 42.850 - 1.870 |
| R-factor | 0.2016 |
| Rwork | 0.200 |
| R-free | 0.23330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6riv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.983 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.970 | 1.930 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmeas | 0.087 | 2.934 |
| Number of reflections | 36297 | 3552 |
| <I/σ(I)> | 5 | 0.7 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.990 | 0.280 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2M sodium chloride, 0.1M MES, 30% v/v Jeffamine ED-2003, pH 6 |
