7ZA4
GSTF sh155 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-09 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9918 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 46.478, 99.003, 153.212 |
| Unit cell angles | 90.00, 93.73, 90.00 |
Refinement procedure
| Resolution | 45.310 - 2.050 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6riv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.914 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.100 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmeas | 0.118 | 2.255 |
| Number of reflections | 43427 | 16765 |
| <I/σ(I)> | 14 | 1 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 4.9 | 4.9 |
| CC(1/2) | 0.998 | 0.456 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 289 | Ammonium sulphate 0.2 M, PEG 4000 15-17.5% w/v |
