7Z8Z

Crystal structure of the MEILB2-BRME1 2:2 core complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-10
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	27.054, 43.992, 178.712
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.680 - 1.500
R-factor	0.1983
Rwork	0.197
R-free	0.23470
Structure solution method	AB INITIO PHASING
RMSD bond length	0.004
RMSD bond angle	0.623
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.360	1.530
High resolution limit [Å]	1.500	1.500
Rpim	0.020	0.309
Number of reflections	34897	1603
<I/σ(I)>	16.7	2.2
Completeness [%]	99.1	94.7
Redundancy	35.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	277	20mM HEPES pH 7.5, 150mM KCl