7Z8Y
Crystal structure of the SUN1-KASH6 9:6 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.999870 |
| Spacegroup name | P 63 |
| Unit cell lengths | 133.762, 133.762, 106.711 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.460 - 2.290 |
| R-factor | 0.1865 |
| Rwork | 0.185 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6r2i |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.731 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.490 | 2.520 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rpim | 0.074 | 0.499 |
| Number of reflections | 32666 | 1635 |
| <I/σ(I)> | 10.6 | 1.8 |
| Completeness [%] | 67.2 | |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M Bis Tris 5.5, 0.3 M magnesium formate, 30% ethylene glycol |
