7Z8E
Crystal structure of the substrate-binding protein YejA from S. meliloti in complex with peptide fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.900, 73.770, 140.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.350 - 1.580 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5icq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.350 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.102 | 1.640 |
| Number of reflections | 86259 | 4780 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 8.8 | |
| CC(1/2) | 0.990 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 292 | 14% PEG 8K, 0.1 M Tris-HCl pH 8.5 0.2 M MgCl2 |
