7Z7Q
Structure of the T207D single-point mutant of the fluorescent protein NeonCyan1 at pH 6.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 50.063, 57.981, 71.446 |
Unit cell angles | 68.57, 89.94, 89.58 |
Refinement procedure
Resolution | 40.060 - 1.600 |
R-factor | 0.2248 |
Rwork | 0.223 |
R-free | 0.25480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ltr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.718 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.060 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmeas | 0.109 | 0.754 |
Number of reflections | 85638 | 8266 |
<I/σ(I)> | 8.8 | 2.4 |
Completeness [%] | 86.7 | 83.6 |
Redundancy | 1.7 | 2.6 |
CC(1/2) | 0.993 | 0.703 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM HEPES pH 6.5 and 22% PEG8000 (w/v) |