7Z7P
Structure of the fluorescent protein NeonCyan0.95 at pH 5.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 |
Unit cell lengths | 66.150, 72.370, 117.360 |
Unit cell angles | 90.19, 90.04, 90.07 |
Refinement procedure
Resolution | 48.900 - 1.950 |
R-factor | 0.2113 |
Rwork | 0.209 |
R-free | 0.25370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ltr |
RMSD bond length | 0.009 |
RMSD bond angle | 1.711 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.900 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmeas | 0.078 | 0.868 |
Number of reflections | 152394 | 15160 |
<I/σ(I)> | 9.7 | 1.7 |
Completeness [%] | 86.7 | 83.6 |
Redundancy | 3.5 | 2.7 |
CC(1/2) | 0.997 | 0.706 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium acetate trihydrate (final pH 5.6), 24% PEG4000 (w/v), 0.2 M ammonium sulphate |