7Z79
Crystal structure of aminotransferase-like protein from Variovorax paradoxus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 164.299, 164.299, 190.419 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 142.290 - 2.300 |
| R-factor | 0.2066 |
| Rwork | 0.205 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cm0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.216 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 190.420 | 190.420 | 2.340 |
| High resolution limit [Å] | 2.300 | 12.600 | 2.300 |
| Rmerge | 0.328 | 0.067 | 3.019 |
| Rmeas | 0.336 | 0.069 | 3.091 |
| Rpim | 0.073 | 0.017 | 0.659 |
| Total number of observations | 2691741 | 15774 | 137294 |
| Number of reflections | 131283 | 915 | 6431 |
| <I/σ(I)> | 7.5 | 38.6 | 0.8 |
| Completeness [%] | 99.7 | 100 | 99.5 |
| Redundancy | 20.5 | 17.2 | 21.3 |
| CC(1/2) | 0.996 | 0.998 | 0.635 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 293 | 0.1 M Citric acid bistris propane pH 4.1, 20% PEG3350 |
