7Z71
Crystal structure of p63 DBD in complex with darpin C14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-03 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 1 |
Unit cell lengths | 53.033, 63.930, 65.504 |
Unit cell angles | 114.46, 94.60, 104.06 |
Refinement procedure
Resolution | 42.690 - 1.850 |
R-factor | 0.1439 |
Rwork | 0.142 |
R-free | 0.18120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qyn 6fpb |
RMSD bond length | 0.016 |
RMSD bond angle | 1.547 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.640 | 44.640 | 1.910 |
High resolution limit [Å] | 1.850 | 7.170 | 1.850 |
Rmerge | 0.034 | 0.024 | 0.110 |
Rmeas | 0.048 | 0.028 | 0.161 |
Rpim | 0.025 | 0.015 | 0.084 |
Number of reflections | 58153 | 995 | 5768 |
<I/σ(I)> | 22 | 9.5 | |
Completeness [%] | 91.6 | 91 | 92.6 |
Redundancy | 3.7 | 3.6 | 3.8 |
CC(1/2) | 0.998 | 0.999 | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 25% PEG 3350, 0.2 M sodium chloride, 0.1 M HEPES pH 7.5 |