7Z6T

Aspergillus clavatus M36 protease without the propeptide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	53.826, 85.037, 55.319
Unit cell angles	90.00, 111.30, 90.00

Refinement procedure

Resolution	45.040 - 1.510
Rwork	0.177
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k90
RMSD bond length	0.013
RMSD bond angle	1.701
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.040	1.564
High resolution limit [Å]	1.510	1.510
Number of reflections	71273	6523
<I/σ(I)>	12.25
Completeness [%]	98.7
Redundancy	6.3
CC(1/2)	0.800	0.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES pH 7.5, 0.2 M lithium sulfate, 25% PEG3350