7Z6N

Crystal structure of Zn2+-transporter BbZIP in a metal-stripped state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-22
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	111.493, 122.117, 101.733
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.170 - 2.570
Rwork	0.288
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tsa
RMSD bond length	0.003
RMSD bond angle	0.724
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_4234)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.170	2.660
High resolution limit [Å]	2.570	2.570
Rpim	0.122
Number of reflections	22269	663
<I/σ(I)>	5.02
Completeness [%]	89.8	29.81
Redundancy	15.5
CC(1/2)	0.999	0.053

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4.5	291	100 mM NaOAc-HOAc pH = 4.5, 100-400 mM LiSO4 and 25-40 % PEG400