7Z6E
Structure of the C1-PH-CNH regulatory module of MRCK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-21 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 |
Unit cell lengths | 69.142, 97.467, 131.413 |
Unit cell angles | 81.38, 77.77, 80.39 |
Refinement procedure
Resolution | 49.600 - 2.140 |
R-factor | 0.1969 |
Rwork | 0.194 |
R-free | 0.24450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AF-O01583-F1 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.941 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.600 | 2.220 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.122 | 1.167 |
Rpim | 0.079 | 0.761 |
Number of reflections | 171288 | 16283 |
<I/σ(I)> | 6.92 | 1.05 |
Completeness [%] | 94.0 | 89.6 |
Redundancy | 3.3 | 3.3 |
CC(1/2) | 0.993 | 0.427 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Bicine/Tris, pH 8.5 30 mM sodium nitrate 30 mM disodium hydrogen phosphate 30 mM ammonium sulfate 20% PEG550 MME 8% PEG20K 3% (v/v) glycerol |