7Z5O

W-formate dehydrogenase from Desulfovibrio vulgaris - Dithionite reduced form

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	64.811, 124.150, 149.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	95.737 - 1.527
Rwork	0.119
R-free	0.15290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sdv
RMSD bond length	0.007
RMSD bond angle	1.415
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.737	1.553
High resolution limit [Å]	1.527	1.527
Number of reflections	182484	9070
<I/σ(I)>	9.8
Completeness [%]	99.6
Redundancy	4.5
CC(1/2)	0.997	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	26% PEG 3350, 0.1 M Tris-HCl pH 8.0 and 1 M LiCl