7Z5K

Transcription factor MYF5 bound to non-symmetrical site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9724
Spacegroup name	C 1 2 1
Unit cell lengths	170.443, 33.839, 53.541
Unit cell angles	90.00, 91.88, 90.00

Refinement procedure

Resolution	45.998 - 2.280
R-factor	0.2309
Rwork	0.229
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mdy
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.000	46.000	2.040
High resolution limit [Å]	1.990	9.100	1.990
Rmerge	0.137	0.096
Rmeas	0.154	0.110
Rpim	0.069	0.052
Total number of observations		1244	1669
Number of reflections	26259	254	801
<I/σ(I)>	3.4	13.8
Completeness [%]	93.1	99.1	55
Redundancy	4.6	4.9	2.1
CC(1/2)	0.996	0.996	0.010

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	295	13% PEG 1000, 2.5% MPD, 6,25% PEG 400, 0.05M Sodium Acetate, pH 4.5