7Z5H
human Zn MATCAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-20 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.280960 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.646, 88.067, 165.581 |
| Unit cell angles | 90.00, 90.77, 90.00 |
Refinement procedure
| Resolution | 47.083 - 2.500 |
| Rwork | 0.232 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7z5g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.978 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.083 | 47.040 | 2.570 |
| High resolution limit [Å] | 2.500 | 10.610 | 2.500 |
| Rmerge | 0.173 | 0.030 | 1.727 |
| Rmeas | 0.204 | 0.036 | 2.038 |
| Rpim | 0.108 | 0.019 | 1.073 |
| Number of reflections | 55542 | 773 | 4531 |
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 100.0 | ||
| Redundancy | 6.8 | 6.5 | 7 |
| CC(1/2) | 0.996 | 0.999 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES-NaOH, 4.3M NaCl, 2 uM ZnCl2, pH 7 |
