7Z4T
AAL160 FAB IN COMPLEX WITH HUMAN INTERLEUKIN-1 BETA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-27 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.795, 37.167, 97.069 |
| Unit cell angles | 90.00, 114.92, 90.00 |
Refinement procedure
| Resolution | 29.340 - 3.300 |
| R-factor | 0.2409 |
| Rwork | 0.238 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i1b |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-2000 (1.9.1) |
| Data scaling software | SCALEPACK (1.6.1) |
| Phasing software | AMoRE (3.4) |
| Refinement software | BUSTER (2.11.8 (20-OCT-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.159 | 0.505 |
| Total number of observations | 38916 | |
| Number of reflections | 9427 | 1032 |
| <I/σ(I)> | 5.28 | 2.5 |
| Completeness [%] | 97.7 | 86.1 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M TRIS pH 8.5, 2.0M ammonium sulfate |
