7Z43
Influenza B polymerase with Pol II pSer5 CTD peptide mimic bound in site 1B and 2B
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-17 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 200.819, 200.819, 257.018 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 76.972 - 3.123 |
| Rwork | 0.240 |
| R-free | 0.27230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5msg |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.171 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.972 | 3.320 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Rmerge | 0.128 | 1.300 |
| Rmeas | 0.149 | 1.490 |
| Number of reflections | 77848 | 3951 |
| <I/σ(I)> | 8 | 1.7 |
| Completeness [%] | 73.1 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.994 | 0.536 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 277 | 200 mM di-ammonium phosphate, and 100 mM sodium acetate at pH 4.4 |
