7Z2J
White Bream virus N7-Methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.25452 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.586, 49.017, 53.763 |
| Unit cell angles | 90.00, 110.04, 90.00 |
Refinement procedure
| Resolution | 39.070 - 1.657 |
| R-factor | 0.1875 |
| Rwork | 0.186 |
| R-free | 0.21460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7z05 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS (20210323) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (3-FEB-2022)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.070 | 39.070 | 1.750 |
| High resolution limit [Å] | 1.657 | 5.240 | 1.660 |
| Rmerge | 0.058 | 0.041 | 0.592 |
| Rmeas | 0.063 | 0.045 | 0.645 |
| Rpim | 0.024 | 0.017 | 0.254 |
| Total number of observations | 5090 | 21942 | |
| Number of reflections | 24275 | 807 | 3508 |
| <I/σ(I)> | 15.3 | 36.4 | 2.7 |
| Completeness [%] | 99.8 | 99.4 | 99.3 |
| Redundancy | 6.6 | 6.3 | 6.3 |
| CC(1/2) | 0.999 | 0.999 | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 293.15 | 0.1 M Tris pH 7.7, 20.3 % PEG 3350, 0.2 M MgCl and 10% isopropanol |
