7Z1Q
X-ray crystal structure of SLPYL1-E151D mutant with NIO molecules
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 87.160, 87.160, 55.276 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.600 - 1.683 |
R-factor | 0.2011 |
Rwork | 0.200 |
R-free | 0.22040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5moa |
RMSD bond length | 0.006 |
RMSD bond angle | 0.873 |
Data reduction software | XDS (VERSION Feb 5, 2021 BUILT=20210205) |
Data scaling software | XDS (VERSION Feb 5, 2021 BUILT=20210205) |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.600 | 1.744 |
High resolution limit [Å] | 1.683 | 1.683 |
Rmerge | 0.043 | 0.964 |
Rmeas | 0.046 | 1.045 |
Rpim | 0.014 | 0.395 |
Number of reflections | 27741 | 2726 |
<I/σ(I)> | 31.29 | 1.91 |
Completeness [%] | 99.8 | |
Redundancy | 9.8 | 6.6 |
CC(1/2) | 0.999 | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | Ammonium sulfate 1.8M |