7Z1P

X-ray crystal structure of SLPYL1-E151D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979261
Spacegroup name	P 32 2 1
Unit cell lengths	89.969, 89.969, 51.768
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.980 - 1.820
R-factor	0.211
Rwork	0.209
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5moa
RMSD bond length	0.007
RMSD bond angle	0.979
Data reduction software	XDS (VERSION Feb 5, 2021 BUILT=20210205)
Data scaling software	XDS (VERSION Feb 5, 2021 BUILT=20210205)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.980	1.885
High resolution limit [Å]	1.820	1.820
Rmerge	0.057	1.979
Rmeas	0.059	2.042
Rpim	0.015	0.498
Number of reflections	21962	2149
<I/σ(I)>	23.84	1.77
Completeness [%]	99.8	99.16
Redundancy	16.4	16.3
CC(1/2)	1.000	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	Ammonium sulfate 1.8M