7Z1E

Nanobody H11-H4 Q98R H100E bound to RBD

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-14
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 31 2 1
Unit cell lengths	78.243, 78.243, 126.812
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.761 - 1.590
Rwork	0.161
R-free	0.20230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zbp
RMSD bond length	0.005
RMSD bond angle	1.306
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.761	1.620
High resolution limit [Å]	1.590	1.590
Rmerge	0.046	4.156
Number of reflections	61104	3027
<I/σ(I)>	24.9	0.4
Completeness [%]	100.0	100
Redundancy	18.8	20.2
CC(1/2)	0.999	0.361

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Ammonium nitrate, 20 % Peg 3350