7Z1C
Nanobody H11-B5 and H11-F2 bound to RBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.900, 56.940, 115.890 |
| Unit cell angles | 80.08, 87.52, 66.11 |
Refinement procedure
| Resolution | 52.000 - 1.900 |
| R-factor | 0.1761 |
| Rwork | 0.175 |
| R-free | 0.19940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zbp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.391 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | |
| Number of reflections | 99469 | 6919 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 96.4 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.998 | 0.504 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 6% v/v Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25% w/v Polyethylene glycol 4,000 |
