7Z0R

AtWRKY18 DNA-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	37.576, 52.182, 81.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.520 - 1.660
R-factor	0.1801
Rwork	0.178
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w1v
RMSD bond length	0.011
RMSD bond angle	1.138
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.520	1.760
High resolution limit [Å]	1.660	1.660
Rmerge	0.068	0.343
Rmeas	0.071
Number of reflections	18612	2274
<I/σ(I)>	24.93	5.68
Completeness [%]	93.5	73.4
Redundancy	12.7	10.2
CC(1/2)	0.999	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	0.03 M sodium nitrate, 0.03 M sodium phosphate, 0.03 M ammonium sulfate, 0.1 M buffer system 3 (Tris (base); BICINE ) pH 8.5, 12.5% v/v MPD; 12.5% PEG 1000 and 12.5% w/v PEG 3350