7Z0G
CPAP:TUBULIN:IE5 ALPHAREP COMPLEX P1 SPACE GROUP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.110, 75.900, 151.320 |
| Unit cell angles | 96.19, 98.28, 111.60 |
Refinement procedure
| Resolution | 48.540 - 3.487 |
| R-factor | 0.2385 |
| Rwork | 0.236 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gwc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.540 | 3.612 |
| High resolution limit [Å] | 3.487 | 3.487 |
| Rmeas | 0.272 | |
| Number of reflections | 28469 | 2508 |
| <I/σ(I)> | 4.96 | |
| Completeness [%] | 96.9 | 84.36 |
| Redundancy | 3.37 | |
| CC(1/2) | 0.982 | 0.394 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M Mes-K, 0.2 M Ca acetate, 20% (V/V) polyethylene glycol 400 |
