7Z0G
CPAP:TUBULIN:IE5 ALPHAREP COMPLEX P1 SPACE GROUP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 |
Unit cell lengths | 57.110, 75.900, 151.320 |
Unit cell angles | 96.19, 98.28, 111.60 |
Refinement procedure
Resolution | 48.540 - 3.487 |
R-factor | 0.2385 |
Rwork | 0.236 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gwc |
RMSD bond length | 0.009 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.540 | 3.612 |
High resolution limit [Å] | 3.487 | 3.487 |
Rmeas | 0.272 | |
Number of reflections | 28469 | 2508 |
<I/σ(I)> | 4.96 | |
Completeness [%] | 96.9 | 84.36 |
Redundancy | 3.37 | |
CC(1/2) | 0.982 | 0.394 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M Mes-K, 0.2 M Ca acetate, 20% (V/V) polyethylene glycol 400 |