7Z0F
CPAP:S-TUBULIN:IIH5 ALPHAREP COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.280, 86.710, 137.610 |
| Unit cell angles | 90.00, 96.61, 90.00 |
Refinement procedure
| Resolution | 45.790 - 2.396 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.27260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gwd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.790 | 2.482 |
| High resolution limit [Å] | 2.396 | 2.396 |
| Rmeas | 0.083 | 1.556 |
| Rpim | 0.054 | |
| Number of reflections | 49614 | 4790 |
| <I/σ(I)> | 13.06 | 1.05 |
| Completeness [%] | 99.2 | 95.8 |
| Redundancy | 4.47 | 4.51 |
| CC(1/2) | 0.998 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.2 M Na tartrate, 10% (W/V) polyethylene glycol 3350 |
