7Z0D

Crystal structure of the L state of bacteriorhodopsin at 1.20 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-19
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9334
Spacegroup name	P 63
Unit cell lengths	60.920, 60.920, 110.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.360 - 1.200
R-factor	0.1547
Rwork	0.154
R-free	0.17390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3w
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.360	29.360	1.230
High resolution limit [Å]	1.200	5.370	1.200
Rmerge	0.057	0.017	1.926
Rmeas	0.061	0.018	2.551
Total number of observations	432771
Number of reflections	69741	817	3901
<I/σ(I)>	20.76	113.43	0.39
Completeness [%]	96.7	97.7	73.7
Redundancy	6.205	9.47	1.633
CC(1/2)	1.000	1.000	0.177

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	Na2HPO4 (5%) and KH2PO4 (95%)