7Z0A

Crystal structure of the ground state of bacteriorhodopsin at 1.22 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-14
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.9334
Spacegroup name	P 63
Unit cell lengths	60.970, 60.970, 109.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.370 - 1.220
R-factor	0.1412
Rwork	0.140
R-free	0.16700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3w
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.370	29.370	1.250
High resolution limit [Å]	1.220	5.440	1.220
Rmerge	0.041	0.014	2.509
Rmeas	0.043	0.016	3.482
Total number of observations	426846
Number of reflections	63006	774	2159
<I/σ(I)>	24.64	130.87	0.3
Completeness [%]	91.2	97	42.4
Redundancy	6.775	9.722	1.395
CC(1/2)	1.000	1.000	0.150

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	Na2HPO4 (5%) and KH2PO4 (95%)