7Z06
Structure of YwlG (Q2FF14) from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-13 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.966 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.741, 136.878, 79.190 |
Unit cell angles | 90.00, 98.55, 90.00 |
Refinement procedure
Resolution | 47.990 - 1.740 |
Rwork | 0.250 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vd8 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.990 | 1.810 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.056 | |
Number of reflections | 114645 | 10476 |
<I/σ(I)> | 10.42 | |
Completeness [%] | 97.8 | 88.8 |
Redundancy | 3.1 | 3.1 |
CC(1/2) | 0.516 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 0.1 M BICINE 1.6 M Ammonium sulfate |