7Z01

Z-SBTubA4 photoswitch bound to tubulin-DARPin D1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	73.468, 91.891, 82.434
Unit cell angles	90.00, 97.40, 90.00

Refinement procedure

Resolution	49.190 - 1.820
R-factor	0.2074
Rwork	0.205
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nqt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.194	2.052
High resolution limit [Å]	1.820	1.825
Number of reflections	43896	2196
<I/σ(I)>	8.7	1.6
Completeness [%]	85.9	70.2
Redundancy	3.5
CC(1/2)	0.998	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Ammonium sulfate, 0.1M Bis/Tris-methane pH=5.5, 21% PEG 3000 (m/v)