7YZG

Crystal structure of the Xenopus FoxH1 bound to the TGTGGATT site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979280
Spacegroup name	P 21 21 21
Unit cell lengths	46.304, 78.726, 103.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.600 - 2.820
R-factor	0.2022
Rwork	0.200
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yz7
RMSD bond length	0.005
RMSD bond angle	0.755
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.600	3.095
High resolution limit [Å]	2.820	2.822
Rpim	0.083
Number of reflections	5547	234
<I/σ(I)>	8.2
Completeness [%]	90.8	68
Redundancy	14.7
CC(1/2)	0.997	0.484

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	30% PEG 8000, 0.2 M ammonium sulfate, 0.1 sodium cacodylate