7YZD

Crystal structure of the zebrafish FoxH1 bound to the TGTTTACT site (fkh motif GTAAACA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	C 2 2 21
Unit cell lengths	36.241, 96.749, 150.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.060 - 2.130
R-factor	0.2131
Rwork	0.211
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yz7
RMSD bond length	0.006
RMSD bond angle	0.776
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.063	2.329
High resolution limit [Å]	2.130	2.144
Rpim	0.043
Number of reflections	9559	478
<I/σ(I)>	11.7
Completeness [%]	63.7	14.9
Redundancy	5.8	4
CC(1/2)	0.998	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	25% PEG Smear High (mix of PEG 6000, PEG 8000, PEG 10000), 0.2 M lithium sulfate