7YZC

Crystal structure of the zebrafish FoxH1 bound to the TGTTTATT site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	36.183, 96.557, 153.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.910 - 2.170
R-factor	0.21202
Rwork	0.210
R-free	0.24136
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yz7
RMSD bond length	0.008
RMSD bond angle	1.233
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.910	2.336
High resolution limit [Å]	2.170	2.172
Rpim	0.055	0.488
Number of reflections	12168	608
<I/σ(I)>	7.9
Completeness [%]	82.5	44.7
Redundancy	6.3
CC(1/2)	0.995	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	20% PEG Smear High (mix of PEG 6000, PEG 8000, PEG 10000), 0.1 M sodium acetate pH 4.5