7YZA

Crystal structure of the zebrafish FoxH1 bound to the TGTGTATT site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	C 1 2 1
Unit cell lengths	99.388, 29.917, 75.719
Unit cell angles	90.00, 108.25, 90.00

Refinement procedure

Resolution	46.780 - 1.180
R-factor	0.1407
Rwork	0.140
R-free	0.14800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yz7
RMSD bond length	0.011
RMSD bond angle	1.652
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.780	1.239
High resolution limit [Å]	1.180	1.180
Rmerge	0.031
Rmeas	0.037
Rpim	0.020	0.627
Number of reflections	57653	3026
<I/σ(I)>	15.4
Completeness [%]	85.1	41.6
Redundancy	3.1	2.5
CC(1/2)	0.999	0.407

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	41% PEG Smear LOW