7YYF
Orthorombic crystal structure of YTHDF1 YTH domain (G459N mutant) form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9717 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.126, 83.289, 123.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.371 - 2.300 |
| R-factor | 0.1791 |
| Rwork | 0.177 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rci |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.939 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.110 | 69.110 | 2.380 |
| High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
| Rmerge | 0.109 | 0.040 | 1.266 |
| Rmeas | 0.114 | 0.042 | 1.319 |
| Rpim | 0.031 | 0.012 | 0.366 |
| Total number of observations | 487980 | 7531 | 46449 |
| Number of reflections | 37685 | 725 | 3635 |
| <I/σ(I)> | 16.3 | 45.7 | 2.2 |
| Completeness [%] | 99.3 | 97.9 | 98.6 |
| Redundancy | 12.9 | 10.4 | 12.8 |
| CC(1/2) | 0.999 | 0.998 | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | 6-12% PEG3350, 0.2 M KSCN |
