7YWE
Monocot chimeric jacalin JAC1 from Oryza sativa: dirigent domain (crystal form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-11 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | H 3 |
Unit cell lengths | 158.900, 158.900, 47.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.870 - 2.150 |
R-factor | 0.2196 |
Rwork | 0.218 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ood |
RMSD bond length | 0.002 |
RMSD bond angle | 0.460 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MoRDa |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.870 | 2.210 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 24426 | 1815 |
<I/σ(I)> | 7.83 | 0.68 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 4.22 | 4.36 |
CC(1/2) | 0.998 | 0.353 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | ammonium dihydrogen phosphate, Tris-HCl, methylpentanediol, dithiothreitol |