7YW2

Crystal structure of tRNA 2'-phosphotransferase from Mus musculus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 31 2 1
Unit cell lengths	65.585, 65.585, 148.459
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.800 - 2.230
R-factor	0.2026
Rwork	0.202
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e3a
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.230	74.230	2.300
High resolution limit [Å]	2.230	8.920	2.230
Rmerge	0.050	0.027	0.567
Rmeas	0.054	0.029	0.606
Rpim	0.019	0.011	0.210
Total number of observations		2442	13662
Number of reflections	34775	362	1693
<I/σ(I)>	22.2	52.4	3.3
Completeness [%]	100.0	99.7	99.6
Redundancy	8.3	6.7	8.1
CC(1/2)	0.999	0.999	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	16% PEG 8000 40 mM Potassium phosphate dibasic 20% Glycerol