7YW1

crystal structure of UBE2O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979136973711562
Spacegroup name	P 21 21 21
Unit cell lengths	115.320, 132.529, 155.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.040 - 3.270
Rwork	0.249
R-free	0.30020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold2 prediction
RMSD bond length	0.002
RMSD bond angle	0.442
Data reduction software	PROCOR
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.740	3.560
High resolution limit [Å]	3.270	3.270
Number of reflections	28617	1431
<I/σ(I)>	9.5
Completeness [%]	92.0
Redundancy	12.8
CC(1/2)	0.996	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283	PEG MME 5000, Bis-Tris