7YVX
Left-handed DNA duplex containing consecutive G-G base pairs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.605 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 43.375, 43.375, 71.998 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.560 - 2.990 |
R-factor | 0.2007 |
Rwork | 0.195 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.560 | 37.560 | 3.160 |
High resolution limit [Å] | 2.990 | 9.470 | 3.000 |
Rmerge | 0.101 | 0.060 | 0.318 |
Rmeas | 0.106 | 0.064 | 0.335 |
Number of reflections | 1553 | 54 | 227 |
<I/σ(I)> | 18.64 | 25.16 | 9.82 |
Completeness [%] | 99.7 | 96.4 | 100 |
Redundancy | 10.003 | 8.333 | 10.282 |
CC(1/2) | 0.998 | 0.998 | 0.988 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | MPD, sodium chloride, hexammine cobalt chloride, sodium cacodylate |