7YVS
Complex structure of Clostridioides difficile binary toxin unfolded CDTa-bound CDTb-pore (short).
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.8 |
Number of Particle | 735102 |
Software | RELION,PHENIX,Coot,cryoSPARC,UCSF Chimera |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |