7YV3
genetically encoded pH sensor Lime at pH10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 98 |
Detector technology | PIXEL |
Collection date | 2019-01-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 109.124, 109.124, 49.663 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.690 - 2.000 |
Rwork | 0.172 |
R-free | 0.20560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vcm |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.960 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.049 | |
Number of reflections | 23179 | 2296 |
<I/σ(I)> | 25.69 | |
Completeness [%] | 99.9 | |
Redundancy | 9.9 | |
CC(1/2) | 1.000 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium phosphate monobasic, 0.1 M Tris pH10, 50% MPD |